Adverse Effects of Pesticides on Agricultural Workers
Assessment of DNA Damage, Oxidative Stress and Serum Mineral Profile in Workers Exposed to Pesticide
Longer exposure to pesticides has harmful health effects and oxidative stress. Due to the production of free radicals and alteration in antioxidant defense enzymes Pesticides cause oxidative stress. Comet assay was used for the evaluation of DNA alterations. Solutions were freshly prepared in the laboratory and fresh blood of pesticide exposed and unexposed individuals were taken to make slides. DNA breakage was significantly higher in occupational pesticide workers. With the increase age and the exposure time DNA breakage increased. The study was done to evaluate the harmful impacts of pesticide on exposed workers.
Biomonitoring of blood biochemical and genotoxic potential of pesticides in exposed rural inhabitants
The 100 blood samples of pesticides exposed peoples have been collected from Bahawalnagar district and total 5ml blood was collected both in EDTA tubes and serum vials for the study of hematological, biochemical and genotoxic potential in pesticides exposed rural inhabitants. Blood sample was collected of different age groups and compared with 100 UN exposed dwellers. Demographic characteristics of pesticides exposed workers have also been observed in the same district. In hematological and biochemical parameters including WBCs, RBCs, hemoglobin, HCT, MCHC, MCH, lymphocytes, MCV, neutrophils, monocytes, eosinophils, potassium, sodium, calcium and phosphorous ,AST, CPK and LDH ,Glucose, Urea, creatinine, Bilirubin, ALT and ALP multiple comparison have done with different diseases and they vary in both pesticide exposed and unexposed individuals.
Biomarkers of oxidative stress and DNA damage among pesticide retailers
This study was conducted to explore the hazardous effects of pesticides on pesticide exposed retailers. For this purpose, blood of total 100 pesticide workers of different age groups were sampled in different areas of district Khanewal Punjab, Pakistan. Comparison was made with 100 non-exposed persons. Analysis of blood samples was done with the help of hematological analyzer and serum collected in this study was use for different biochemical tests. Hematological parameters including HGB, RBCs count, hematocrit, MCV, MCHC, monocytes, lymphocytes, neutrophils and eosinophils vary in different diseases in the exposed and unexposed workers. The percentages of occurrence of certain diseases were high in exposed group than the unexposed groups. Biochemical parameters ALT, AST, LDH, CPK, ALP, Creatinine, urea, lipid profile tests, and tests of certain minerals showed variations of values in different diseases. The comet assay is used to check the DNA damage in lymphocytes of both exposed and unexposed groups.
Investigation of Various Adverse Effects of Pesticides in Exposed Workers
This study was conducted to investigate the toxic effects of pesticides on pesticide exposed workers. For this purpose total 100 workers of different age groups were sampled from different districts of Punjab, Pakistan. They were compared with 100 healthy non-exposed persons. Workers blood, serum and urine samples were collected. Blood samples were analyzed by using hematological analyzer and serum collected in this study use for different serological tests. Urine was also use for urine tests. Hematological parameters including hemoglobin, erythrocytes count, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, platelets were non-significant when compared with non-exposed persons but total leucocyte count, neutrophils and eosinophils were significantly increased. Lymphocytes and monocytes were significantly decreased. Serological parameters alanine transaminase, aspartate transaminase and alkaline phosphatase were significantly increased. Total protein, albumin and globulin were significantly decreased.